Submit this document into the appropriate LC assigment folder by the due date. Respond to the questions by typing directly into this document. How to perform assigment: Read through this activity sheet and respond to the questions/prompts underneth Calculations involved in agarose gel preparation.
Purpose of assignment: The purpose of this activity is to give you practice performing the calculations involved with agarose gel electrophoresis assay including and buffer and sample preparation. The calulations performed in this activity are located at to the beginning wherase in the actual in-class activity they are scattered throughout. A big difference between this activity and the actual activity are the calculations that msut be performed. This activity is formatted very similarly to the actual in-class activity. Every time you run an agarose gel electrophoresis assay on your DNA samples, you should always include a DNA ladder as a refeerce sample next to your samples to help data interpreration. DNA ladders are referred to as a reference sample because it is used to help you understand your data. In this make-up lab assignment, you will perform the calculations necessary to prepare for and run an gel electrophoresis assay on a special sample called a DNA ladder.
For your education, the protocol the students are following for the in-class activity is colored in blue. However, this make-up assignment will focus on 1, preparing for agarose gel electrophoresis. That actual in-class activity asks each student to 1) prepare for agarose gel electrophoresis by performing the calculations necessary and 2) using the calculations and applying them to a protocol. The framework of this assignment is similar to the actual in-class activity that would be encountered.
Synopsis: This make-up lab assignment will correspond more to the other in-class activity, agarose gel electrophoresis. The make-up lab will focus on agarose gel electrophoresis rather than EndNote because of the EndNote, a student should feel welcome to try and download this program (available from UGA library) and perform the assignment at their leisure, as this would be very beneficial to their classroom research project. Transcribed image text: of 9-2 through 9-10 About this class period: The major topics for the second F2F (face-to-face) class period of the semester, from 9-2 software, EndNote.
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